Coding

Part:BBa_K4410004:Design

Designed by: Ruyi Shi   Group: iGEM22_Worldshaper-HZBIOX   (2022-09-21)


argJ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 247
    Illegal PstI site found at 932
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 697
    Illegal PstI site found at 247
    Illegal PstI site found at 932
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 247
    Illegal PstI site found at 932
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 247
    Illegal PstI site found at 932
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

argJ gene encodes bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase . bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase converts L-glutamate into N-acetyl-L-glutamate and can function well at high concentration of arginine. argJ gene is designed to be introduced into Escherichia coli to replace the role of inactivated NAGS at high concentration of arginine in the acetylation of glutamate. The acetylation of glutamate is the first step of arginine biosynthesis in E. coli.


Source

The source of the part has been deposited in GenBank under the accession number CGL_RS06970.

References